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1-Deoxynojirimycin Carbohydrate Metabolism modulator

Name: 1-Deoxynojirimycin
Brand: Selleck Chemicals
SKU: S3839
Rating: 5 (1 reviews)

Cat.No.S3839

1-Deoxynojirimycin (duvoglustat, moranolin) is a potent α-glucosidase inhibitor and most commonly found in mulberry leaves. It has therapeutic potency against diabetes mellitus.

Chemical Structure

Molecular Weight: 163.17

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Quality Control

Batch: Purity: 98.33%

98.33

Related Targets	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Mitochondrial Metabolism Drug Metabolite
Other Carbohydrate Metabolism Inhibitors	2-DG (2-Deoxy-D-glucose) Bromopyruvic acid (3-BP) Lonidamine Dorzagliatin LY2608204 Voglibose 4',7-Dimethoxy-5-Hydroxyflavone AZD1656 Nodakenetin Kaempferol-3-O-neohesperidoside

Chemical Information, Storage & Stability

Molecular Weight	163.17	Formula	C₆H₁₃NO₄	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	19130-96-2	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	duvoglustat, moranolin			Smiles	C1C(C(C(C(N1)CO)O)O)O

Solubility

In vitro
Batch:

DMSO : 33 mg/mL (202.24 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 33 mg/mL

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.650mg/ml (10.11mM)	Taking the 1 mL working solution as an example, add 50 μL of 33 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.270mg/ml (1.65mM)	Taking the 1 mL working solution as an example, add 50 μL of 5.4 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	α-glucosidase (Cell-free assay)
In vitro	1-Deoxynojirimycin (DNJ) up to 200 μmol/L does not influence survival of HUVECs. This compound behaves as an antioxidant, decreases ROS production, and delays cellular senescence.
In vivo	1-Deoxynojirimycin (DNJ) inhibits intestinal glucose absorption in intestine. This compound down-regulates intestinal SGLT1, Na+/K+-ATP and GLUT2 mRNA and protein expression. Pretreatment with this chemical (50 mg/kg) increases the activity, mRNA and protein levels of hepatic glycolysis enzymes (GK, PFK, PK, PDE1) and decreases the expression of gluconeogenesis enzymes (PEPCK, G-6-Pase), improves glucose tolerance in both normal and diabetic mice. It inhibits intestinal glucose absorption and accelerates hepatic glucose Metabolism by directly regulating the expression of proteins involved in glucose transport systems, glycolysis and gluconeogenesis enzymes. DNJ reduces oxidative stress in the liver and plasma in rodents.
References	[1] https://pubmed.ncbi.nlm.nih.gov/23536174/ [2] https://pubmed.ncbi.nlm.nih.gov/24709343/ [3] https://pubmed.ncbi.nlm.nih.gov/11752220/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT01380743	Completed	Pompe Disease	Amicus Therapeutics	October 31 2011	Phase 2

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