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Z-FA-FMK Cysteine Protease inhibitor

Name: Z-FA-FMK
Brand: Selleck Chemicals
SKU: S7391
Rating: 5 (11 reviews)

Cat.No.S7391

Z-FA-FMK ((1S)-Z-FA-FMK) is an irreversible cysteine protease inhibitor, and also inhibits effector caspases.

Chemical Structure

Molecular Weight: 386.42

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Quality Control

Batch: Purity: 99.69%

99.69

Related Targets	HDAC Caspase Proteasome Secretase MMP HCV Protease DPP Tyrosinase HIV Protease Serine Protease
Other Cysteine Protease Inhibitors	N-Ethylmaleimide (NEM) MG132 SSS Calpeptin Aloxistatin (E-64d) Odanacatib E-64 MG-101 (ALLN) Cathepsin Inhibitor 1 PD 151746 Loxistatin Acid (E-64C)

Chemical Information, Storage & Stability

Molecular Weight	386.42	Formula	C₂₁ H₂₃ F N₂ O₄	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	197855-65-5	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	(1S)-Z-FA-FMK			Smiles	CC(C(=O)CF)NC(=O)C(CC1=CC=CC=C1)NC(=O)OCC2=CC=CC=C2

Solubility

In vitro
Batch:

DMSO : 77 mg/mL (199.26 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 48 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	cysteine protease
In vitro	Z-FA-FMK inhibits the degradation of fibrillar collagen by fibroblasts and osteoclasts. This compound inhibits LPS-induced cytokine production via inhibition of NF-kappaB-dependent gene expression in macrophages. It efficiently blocks T cell proliferation induced by mitogens and IL-2 in vitro.
In vivo	Z-FA-FMK significantly increases pneumococcal growth in both lungs and blood in a mouse model of intranasal pneumococcal infection. This compound blocks reovirus infection of Ras oncogenic tumours and host heart tissues in severe combined immunodeficiency mice.
References	[1] https://pubmed.ncbi.nlm.nih.gov/4073501/ [2] https://pubmed.ncbi.nlm.nih.gov/2069557/ [3] https://pubmed.ncbi.nlm.nih.gov/11290751/ [4] https://pubmed.ncbi.nlm.nih.gov/16951345/ [5] https://pubmed.ncbi.nlm.nih.gov/20834102/

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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