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MDL-28170 Secretase inhibitor

Name: MDL-28170
Brand: Selleck Chemicals
SKU: S7394
Rating: 5 (9 reviews)

Cat.No.S7394

MDL-28170 (Calpain Inhibitor III) is a potent and selective calpain inhibitor of calpain that penetrates the blood-brain barrier and inhibits brain cysteine protease activity after systemic administration. This compound is also an inhibitor of γ-secretase.

Chemical Structure

Molecular Weight: 382.45

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Quality Control

Batch: Purity: 99.26%

99.26

Related Targets	HDAC Caspase Proteasome MMP HCV Protease Cysteine Protease DPP Tyrosinase HIV Protease Serine Protease
Other Secretase Inhibitors	DAPT RO4929097 (RG-4733) LY411575 Nirogacestat (PF-03084014) Dibenzazepine (YO-01027) Avagacestat (BMS-708163) Semagacestat (LY450139) MK-0752 L-685,458 NGP 555

Chemical Information, Storage & Stability

Molecular Weight	382.45	Formula	C₂₂H₂₆N₂O₄	Storage (From the date of receipt)	2 years -80 in solvent
CAS No.	88191-84-8	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Calpain Inhibitor III			Smiles	CC(C)C(C(=O)NC(CC1=CC=CC=C1)C=O)NC(=O)OCC2=CC=CC=C2

Solubility

In vitro
Batch:

DMSO : 78 mg/mL (203.94 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 6 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.700mg/ml (7.06mM)	Taking the 1 mL working solution as an example, add 50 μL of 54 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	calpain Secretase
In vitro	MDL-28170 significantly enhances Schwann cells (SCs) survival in vitro in response to oxidative stress induced by application of H₂O₂, without reducing LDH release.
In vivo	MDL 28170 is a calpain inhibitor that protects against cortical neuronal damage even if the treatment is delayed until 3 h after reperfusion. However, the neuroprotective effect of this compound is less pronounced in the hippocampal CA1 sector.
References	[1] https://pubmed.ncbi.nlm.nih.gov/9637399/ [2] https://pubmed.ncbi.nlm.nih.gov/24884926/ [3] https://pubmed.ncbi.nlm.nih.gov/20568964/

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Handling Instructions

Tel: +1-832-582-8158 Ext:3

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