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Gamma-Oryzanol DNA Methyltransferase inhibitor

Name: Gamma-Oryzanol
Brand: Selleck Chemicals
SKU: S3957
Rating: 5 (3 reviews)

Cat.No.S3957

Gamma-Oryzanol (GO), a nutriactive phytochemical naturally occurring in crude rice bran oil, is an antioxidant compound used to treat hyperlipidaemia, disorders of menopause and to increase muscle mass. γ-Oryzanol is a potent DNA Methyltransferases (DNMTs) inhibitor in the striatum of mice. γ-Oryzanol significantly inhibits the activities of DNMT1 (IC50=3.2 μM), DNMT3a (IC50=22.3 μM).

Chemical Structure

Molecular Weight: 602.89

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Quality Control

Batch: S395701 DMSO]100 mg/mL]false]]]false]]]false Purity: 96.54%

Cited in Nature Medicine for its top-tier quality
COA
HPLC
SDS
Datasheet

96.54

Related Targets	HDAC JAK BET Histone Methyltransferase PKC PARP HIF PRMT EZH2 AMPK
Other DNA Methyltransferase Inhibitors	RG108 SGI-1027 Zebularine (NSC 309132) GSK3685032 CM272 β-thujaplicin Bobcat339 DC-05 2'-Deoxy-5-Fluorocytidine GSK-3484862

Chemical Information, Storage & Stability

Molecular Weight	602.89	Formula	C₄₀H₅₈O₄	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	11042-64-1	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	CC(CCC=C(C)C)C1CCC2(C1(CCC34C2CCC5C3(C4)CCC(C5(C)C)OC(=O)C=CC6=CC(=C(C=C6)O)OC)C)C

Solubility

In vitro
Batch:

DMSO : 100 mg/mL (165.86 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

In vitro	From the proliferation assay using MTT reagent, the effect of Gamma-Oryzanol on DU145 and PC3 are slightly different with an IC50 after 7 days of exposure at 0.52±0.06 and 0.40±0.08 mg/ml, respectively. For in vitro conditions in proliferation assay, IC50 values seem to be high in the scale of mg/ml. Gamma-Oryzanol may affect prostate cancer cells through the down regulation of some antioxidant genes, CAT and GPX.
In vivo	In tumour-bearing mice, Gamma-Oryzanol can reduce tumour mass associated with pro-angiogenic biomarkers. Gamma-Oryzanol is not carcinogenic and is safe for animals. In vivo trials proved Gamma-Oryzanol to be a powerful antioxidant and also appears to be a very promising anticancerogenic compound. Supplementation of Gamma-Oryzanol in diabetic patients may improve metabolic dysfunctions such as hyperglycaemia, hypercholesterolaemia, hypertriglyceridaemia, insulin resistance and may alleviate diabetic complications resulting from lipid peroxidation and free radicals.
References	[1] https://pubmed.ncbi.nlm.nih.gov/24175837/ [2] https://pubmed.ncbi.nlm.nih.gov/18424018/ [3] https://pubmed.ncbi.nlm.nih.gov/26718022/

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Handling Instructions

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