research use only

TLQP-21 TFA

Cat.No.P1209

LQP-21 TFA (TLQP-21 Trifluoroacetate) is a VGF-derived peptide that activates the C3aR1 receptor through a folding-upon-binding mechanism.

Amino-acid Sequence

CAS No. 869988-94-3(free base)

Quality Control

Batch: Purity: 99.90%

99.90

Storage (from the date of receipt)	3 years-20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
			1 month-20°Cin solvent

In vitro
Batch:

DMSO : 100 mg/mL (39.26 mM)；
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 100 mg/mL

Ethanol : 100 mg/mL

In Vivo
Batch:

Add solvents to the product individually and in order.

Description	LQP-21 TFA (TLQP-21 Trifluoroacetate) is a VGF-derived peptide that activates the C3aR1 receptor through a folding-upon-binding mechanism.
Targets/IC₅₀/Ki	C3aR1 receptor

In vitro
In vitro	TLQP-21 activates the cognate C3aR1 receptor leading to enhanced lipolysis in adipocytes and arginine 21 is essential for TLQP-21 lipolysis in 3T3L1 adipocytes.
Cell Research	Cell Lines	3T3L1 adipocytes
	Concentrations	100 nM
	Incubation Time	3 h
	Method	3T3-L1 adipocytes differentiated on 6-well plates are first starved for 3 hours in KRH with 1% BSA, then incubate with TLQP-21 in the presence and absence of specific agonists/antagonists, suspended in KRH with 4% BSA. Following 3 hours of incubation at 37°C and 5% CO2, the media is collected and incubated at 60°C for 20 min to inactivate any residual enzymatic reaction activity. The cells are gathered for protein concentration or trypsinized for cell diameter and viability measurements.

References
https://pubmed.ncbi.nlm.nih.gov/25456411/

Molecular Formula	C₁₀₇H₁₇₀N₄₀O₂₆(TFA)
Molecular Weight	2546.75
CAS No.	869988-94-3(free base)
Appearance	White Powder
Peptide Content (by %N)	≥80%
Single Impurity (by HPLC)	≤1.0%
Assay (By Anhydrous, Acetic Acid-free)	95.0~105.0%
MS	Consistent
Packing	plastic vial(dedicated for peptide packing) or glass vial, quantity according to customer's detail requirement.
Storage (From the date of receipt)	3 years-20°C powder
Shipping	Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)