research use only

Desmopressin Acetate

Cat.No.P1084

Desmopressin is a synthetic octapeptide, and an analogue of human hormone arginine vasopressin with antidiuretic and coagulant activities. It is a selective agonist for the vasopressin V2 receptor (V2R).

Amino-acid Sequence

CAS No. 62288-83-9

Quality Control

Batch: Purity: 99.02%

99.02

Chemical Information & Solubility

Storage (from the date of receipt)	3 years-20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
			1 month-20°Cin solvent

In vitro
Batch:

DMSO : 100 mg/mL (88.55 mM)；
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 100 mg/mL

Ethanol : 100 mg/mL

Molecular Weight Calculator

In Vivo Batch: Add solvents to the product individually and in order.					In vivo Formulation Calculator
	Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.	In vivo Formulation Calculator
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	5.000mg/ml (4.43mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil	0.625mg/ml (0.55mM)	Taking the 1 mL working solution as an example, add 50 μL of 12.5 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

Biological Activity

Description	Desmopressin is a synthetic octapeptide, and an analogue of human hormone arginine vasopressin with antidiuretic and coagulant activities. It is a selective agonist for the vasopressin V2 receptor (V2R).

In vitro
In vitro	Desmopressin (DDAVP) is a selective agonist for the Vasopressin V2 receptor (V2R), which is coupled to cAMP-dependent signalling. DDAVP induces cAMP-dependent eNOS activation via Ser1177 phosphorylation. DDAVP (at doses ranging from 100 ng/ml to 1 μg/ml) exerts a modest but significant antiproliferative effect on cultured CT-26 and Colo-205 cells.
Cell Research	Cell Lines	HUVECs
	Concentrations	1 μM
	Incubation Time	25 min
	Method	HUVECs are transduced with recombinant adenoviruses AdV2R.EGFP (AdV2R) or AdlacZ. 3 days later cells are incubated for 25 min with 1 μM DDAVP or 10 μM forskolin, both in presence of IBMX. Cell extracts are analyzed for eNOS Ser1177 phosphorylation and eNOS expression.

In Vivo
In Vivo	In vivo, DDAVP (2 μg/kg) reduces accumulation of ascites and formation of intestinal tumour nodules in mice intraperitoneally inoculated with CT-26 cells. Perioperative administration of DDAVP significantly inhibits tumour progression in animals surgically implanted in the spleen with CT-26 cells, and causes some reduction in liver metastasis. DDAVP is able to inhibit lung colonisation by blood-borne breast cancer cells in an experimental murine model. It dramatically reduces lymph node and lung metastasis in a model of mammary tumour manipulation and surgical excision in mice when administered perioperatively, and also exerts anti-tumour effects in combination with chemotherapy. DDAVP increases the plasma levels of coagulation factor VIII, von Willebrand factor (VWF) and tissue-type plasminogen activator, and also enhances platelet adhesion to the vessel wall.
Animal Research	Animal Models	Syngeneic adult male Balb/c mice inoculated intraperitoneally or intrasplenically with CT-26 colon cancer cells
	Dosages	2 μg/kg body weight
	Administration	i.v.

References
https://pubmed.ncbi.nlm.nih.gov/12871421/ https://pubmed.ncbi.nlm.nih.gov/12871421/

References

https://pubmed.ncbi.nlm.nih.gov/12871421/
https://pubmed.ncbi.nlm.nih.gov/12871421/

Product Information

Molecular Formula	C₄₆H₆₄N₁₄O₁₂S₂.C₂H₄O₂
Molecular Weight	1129.27
CAS No.	62288-83-9
Appearance	White Powder
Peptide Content (by %N)	≥80%
Single Impurity (by HPLC)	≤1.0%
Assay (By Anhydrous, Acetic Acid-free)	95.0~105.0%
MS	Consistent
Packing	plastic vial(dedicated for peptide packing) or glass vial, quantity according to customer's detail requirement.
Storage (From the date of receipt)	3 years-20°C powder
Shipping	Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)