STF-118804

Catalog No.S7316 Batch:S731601

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Technical Data

Formula

C25H23N3O4S
Molecular Weight 461.53 CAS No. 894187-61-2
Solubility (25°C)* In vitro DMSO 61 mg/mL (132.16 mM)
Water Insoluble
Ethanol Insoluble
In Vivo (Add solvents to the product individually and in order.)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 1.000mg/ml (2.17mM) Taking the 1 mL working solution as an example, add 50 μL of 20 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 0.300mg/ml (0.65mM) Taking the 1 mL working solution as an example, add 50 μL of 6 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description STF-118804 is a highly specific NAMPT inhibitor.
Targets
NAMPT
In vitro STF-118804 reduces the viability of B-ALL cell lines containing MLL chromosomal translations, with IC50 values in the low nanomolar range. In addition, leukaemic samples from five paediatric ALL patients are also sensitive to this compound in the low nanomolar range. This chemical induces leukaemia MV411 cell apoptosis without antecedent cell cycle arrest.
In Vivo STF-118804 (25 mg/kg twice daily, s.c.) improves survival in an orthotopic xenotransplant model of high-risk acute lymphoblastic leukaemia, and effectively depletes leukaemia-initiating cells.

Protocol (from reference)

Kinase Assay:[1]
  • Enzyme Assays

    The enzymatic activities of NAMPT and NMNAT are measured using in vitro kits and using the Two-Step Method per manufacturer’s instructions. Compounds, NAMPT and/or NMNAT enzymes, and their substrates are mixed and incubated at 30°C for 1 hour. Reagents for the indicator reaction (Wst-1) are then added, and absorbance is read at 450 nm every 5 min at 30°C on a Tecan Infinite M100 multimode plate reader.
Cell Assay:[1]
  • Cell Lines

    Human cell lines (SEM, MV411, Nalm6, 697, Hal01, REH, SUP-B15 and KP-Y-RL) and primary patient leukemic samples

  • Concentrations

    ~10 μM

  • Incubation Time

    72 hours

  • Method

    Human cell lines or lineage negative cord blood cells were seeded into 96-well plates (6×105 cells per milliliter). Compounds are added in increasing concentrations, and cells are incubated at 37°C/5% CO2 for 72 hours. To detect viability, CellTiter-Blue reagent is added at 1:10 dilution, and plates are incubated for 4 hours at 37°C/5% CO2 prior to reading on a Flexstation II 384 or a Synergy H1 reader at an excitation of 555 nm and emission detection of 590 nm. Cell viability is also measured by CellTiter-Fluor. The cell-permeable fluorogenic peptide substrate GF-AFC reagent is added at 1:2 dilution, and plates are incubated for 30 min at 37°C/5% CO2 prior to reading on a Synergy H1 reader at an excitation of 380 nm and emission detection of 505 nm. Cord blood cells are enumerated on a hemocytometer, and cell viability is assessed with trypan blue exclusion dye. Inhibitory concentration (IC50) is calculated using Prism software. Primary patient samples are plated in 96-well plates and treated with increasing concentrations of this compound for 48 hours at 37°C in 5% CO2. WST-1 reagent is added to the culture medium (1:10 dilution), and absorbance is measured at 450 nm using a Bio-Rad model 680 microplate reader. All assays are performed in triplicate. IC50 is calculated using CalcuSyn version 2.0 software.
Animal Study:[1]
  • Animal Models

    Orthotopic xenograft model of ALL transplanted with MV411 cells

  • Dosages

    25 mg/kg twice daily

  • Administration

    s.c.

References

  • https://pubmed.ncbi.nlm.nih.gov/24183972/

Customer Product Validation

RSV-induced SIRT1 expression is PARP1-dependent in PC12 cells. a, PC12 cells were pretreated with STF-118804 (100 nM) for 1 h prior to treatment with RSV (20 lM) for another 2 h, followed bytreatment with or without of Ab25–35 (30 lM) for an additional 24 h. SIRT1 expression was determined by western blot analysis. b, The bar graph shows the quantification of endogenous SIRT1. c, Cells were treated with various concentrations (1, 5, 10, and 20 lM) of RSV for 24 h. PARP1 expression was detected by western blot analysis.

Data from [ , , Neurochem Res, 2016, 41(9):2367-79. ]

Sellecks STF-118804 Has Been Cited by 6 Publications

A skin organoid-based infection platform identifies an inhibitor specific for HFMD [ Nat Commun, 2025, 16(1):2513] PubMed: 40082449
Efficacy of NAMPT inhibition in T-cell acute lymphoblastic leukemia [ PLoS One, 2025, 20(6):e0324443] PubMed: 40526635
Ultrasensitive sensors reveal the spatiotemporal landscape of lactate metabolism in physiology and disease [ Cell Metab, 2022, S1550-4131(22)00453-3] PubMed: 36309010
NAD+ supplement potentiates tumor-killing function by rescuing defective TUB-mediated NAMPT transcription in tumor-infiltrated T cells [ Cell Rep, 2021, 36(6):109516] PubMed: 34380043
Bacteria Boost Mammalian Host NAD Metabolism by Engaging the Deamidated Biosynthesis Pathway. [ Cell Metab, 2020, 31(3):564-579] PubMed: 32130883
Resveratrol Attenuates Aβ25-35 Caused Neurotoxicity by Inducing Autophagy Through the TyrRS-PARP1-SIRT1 Signaling Pathway [Deng H, et al. Neurochem Res, 2016, 41(9):2367-79] PubMed: 27180189

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.