In Vivo (Add solvents to the product individually and in order.)
Homogeneous suspension
CMC-NA
≥5mg/ml
Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO
40%PEG300
5%Tween80
50%ddH2O
Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.
2.500mg/ml
(3.93mM)
Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution
5% DMSO
95% Corn oil
Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.
1.250mg/ml
(1.96mM)
Taking the 1 mL working solution as an example, add 50 μL of 25 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)
Preparing Stock Solutions
Biological Activity
Description
NSC348884, as a nucleophosmin inhibitor, inhibit cell proliferation and induce apoptosis in various cancer cell lines with IC50 values ranging from 1.4-4 µM.
Targets
nucleophosmin (In Granta cells)
nucleophosmin (In LNCaP cells)
1.7 μM
4 μM
In vitro
NSC348884 is a putative nucleophosmin small molecular inhibitor that disrupts a defined hydrophobic pocket required for oligomerization. It disrupts nucleophosmin oligomer formation by native polyacrylamide gel electrophoresis assay. This compound upregulates p53 and induces apoptosis.
In Vivo
In vivo invasion and intravasation (that is, the number of CTCs) are significantly inhibited after injection of NSC348884 (as an inhibitor of NPM1 oligomerization) into the tumor-bearing mice. No significant difference in overall cell death is observed by histology in the treated tumors with the 4-hour brief treatments, suggesting that the inhibition seen is specific to migration..
Mice bearing either orthotopic MDA-MB-231-GFP tumors (MDA231) or patient-derived HT17 and HT19 tumors
Dosages
25 mg/kg
Administration
i.p.
References
https://pubmed.ncbi.nlm.nih.gov/18345031/
https://pubmed.ncbi.nlm.nih.gov/23113900/
Sellecks NSC348884 Has Been Cited by 6 Publications
The nucleolar granular component mediates genome-nucleolus interactions and establishes their repressive chromatin states
[ Mol Cell, 2025, S1097-2765(25)00409-5]
The EGFR/ErbB inhibitor neratinib modifies the neutrophil phosphoproteome and promotes apoptosis and clearance by airway macrophages
[ Front Immunol, 2022, 13:956991]
RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.
