Cetrimonium Bromide (CTAB)

Catalog No.S4242 Batch:S424201

Technical Data

Formula

C₁₉H₄₂N.Br

Molecular Weight

364.45

CAS No.

57-09-0

Solubility (25°C)*

In vitro

Water

100 mg/mL (274.38 mM)

Ethanol

100 mg/mL (274.38 mM)

DMSO

5 mg/mL (13.71 mM)

In Vivo (Add solvents to the product individually and in order.)

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.25mg/ml (0.69mM)	Taking the 1 mL working solution as an example, add 50 μL of 5 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.25mg/ml (0.69mM)	Taking the 1 mL working solution as an example, add 50 μL of 5 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Cetrimonium Bromide (CTAB) is a known component of the broad-spectrum antiseptic cetrimide, which is a mixture of different quaternary ammonium salts.
In vitro	Cetrimonium Bromide (CTAB) reduces cell viability of FaDu, C666-1, UTSCC-8A, UTSCC-42A, MRC5 cells and GM05757 fibroblasts with EC50 of 2 μM, 3.8 μM, 3.5 μM, 4.2 μM, 11 μM and 18 μM, respectively, and of A549 lung and MCF7 breast cancer cells with EC50 of 17 μM and 12 μM. It (0.5 μM) interacts additively with radiation in a dose-dependent manner to exhibit inhibition effect on the clonogenic survival of FaDu cells. This compound results in nuclear condensation and blebbing in FaDu cells, consistent with apoptotic nuclear morphology, which is not observed in CTAB-treated GM05757 fibroblasts. It reproducibly decreases ATPase activity in a dose-dependent manner in FaDu cells, achieving ∼90% inhibition at 50 μM. At 5 μM, it causes a modest (∼10%), but statistically significant decreases in ATP content in FaDu cells. Its induced apoptosis (5 μM) is significantly suppressed by >50% when CCCP (5 μM) is used to effectively uncouple ΔΨM before CTAB treatment in FaDu cells.
In Vivo	Cetrimonium Bromide (CTAB) (5 μM) effectively eliminates the tumour-forming potential of FaDu cells in SCID mice, and this compound (daily 5 mg/kg i.p. for 5 days) induces a modest reduction in tumour development compared with the vehicle-treatment arm in established FaDu xenograft tumours in SCID mice.

Protocol (from reference)

References

https://pubmed.ncbi.nlm.nih.gov/19654225/

Sellecks Cetrimonium Bromide (CTAB) Has Been Cited by 2 Publications

Cetrimonium bromide promotes lipid clearance via TFEB-mediated autophagy-lysosome activation in hepatic cells [ Biochem Cell Biol, 2021, 10.1139/bcb-2020-0570]	PubMed: 33560918
Highly Sensitive Label-Free Detection of Small Molecules with an Optofluidic Microbubble Resonator [Li Z, et al. Micromachines (Basel), 2018, 9(6)]	PubMed: 30424207

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.