In Vivo (Add solvents to the product individually and in order.)
Homogeneous suspension
CMC-NA
≥5mg/ml
Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO
40%PEG300
5%Tween80
50%ddH2O
Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.
4.15mg/ml
(9.97mM)
Taking the 1 mL working solution as an example, add 50 μL of 83 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution
5% DMSO
95% Corn oil
Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.
0.59mg/ml
(1.42mM)
Taking the 1 mL working solution as an example, add 50 μL of 11.8 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)
Preparing Stock Solutions
Biological Activity
Description
Daidzin (Daidzoside, Daidzein 7-O-glucoside, Daidzein 7-glucoside), a natural organic compound in the class of phytochemicals known as isoflavones, is a potent and selective inhibitor of human mitochondrial aldehyde dehydrogenase and inhibits ALDH-I selectively (Ki=20 nM); at least 500 times less effective against ALDH-Ⅱ, the cytosolic isozyme (Ki=10 μM).
Targets
ALDH-Ⅰ (Cell-free assay)
20 nM(Ki)
In vitro
Daidzin, a glycoside of daidzein, increases the transcriptional activity of RARα and RARγ but does not bind to the RARs. This compound does not inhibit human class Ⅰ, Ⅱ, or Ⅲ alcohol dehydrogenases, nor does it have any significant effect on biological systems that are known to be affected by other isoflavones. It inhibits human ALDH-I and ALDH-II in a concentration-dependent manner. This chemical inhibits both ALDH-I and ALDH-II in an apparently competitive manner with Ki values of 40 nM and 20 μM, respectively, and it inhibits ALDH-I uncompetitively with respect to NAD+. The inhibition of ALDH-I by daidzin is reversible.
In Vivo
Daidzin has no effect on alcohol-metabolising enzymes (i.e., ADH and ALDH) when given to rats intragastrically. Chronic administration of this compound exerts an effect on alcohol pharmacokinetics, although the effect is less pronounced than when it is administered concurrently with ethanol. It is shown to shorten sleep time if ethanol is given intragastrically, but not when given intraperitoneally, indicating absence of effect on ethanol elimination rate. This chemical delays ethanol absorption and lessens alcohol intoxication. It is shown to suppress the levels of BAC (blood alcohol concentration) for the first 3 hr after alcohol ingestion in both fasted and fed rats. These effects may in part be due to its antioxidant activity.
CV-1 cells are transfected with 100 ng ERE-Luc and 50 ng ER-RARα or ER-RARγ. Transfected cells are treated with the indicated isoflavone at 0.5, 1, 10, 50, and 100 μM for 24 h. The β-Gal activity is used to normalize luciferase activity.
Daidzin improves the cisplatin chemosensitivity for osteosarcoma via binding to β-catenin protein and suppressing the wnt pathway
[ Sci Rep, 2025, 15(1):5484]
RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.
